Human TNFR1/CD120a/TNFRSF1A Protein, hFc TagSpecifications Synonyms TNFR I; TNF RI; TNF R1; CD120a Source Human TNFR1 CD120a TNFRSF1A Protein is expressed from HEK293 with hFc tag at the C Terminus. It contains Leu30 Thr211.[Accession P19438 1] Molecular Weight The protein has a predicted MW of 48 kDa. Due to glycosylation, the protein migrates to 60 70 kDa based on SDS PAGE result. Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS PAGE and HPLC. Activity ELISA

② Reasonable application of additives

a 26 aa transmembrane segment

All three IFN-lambdas use a distinct receptor system composed of an IFN-λR1 subunit (also called CRF2-12) and IL-10R2 subunit (also called CRF2-14)

coli-derived Human GDF-5/BMP-14

causing the color of the reaction solution to change from blue-purple to sky-blue

the protein migrates to 90-100 kDa based on Tris-Bis PAGE result

dilute 2 μg of target DNA with 100 μL of serum-free medium

Primary cells/ Immune cells/ Epithelial cells and other cells

G protein coupled receptor 75

Human CCL2 cDNA encodes a 99 amino acid (aa) precursor protein with a 23 aa signal peptide and a 76 aa mature protein

2） Quantitative system: add 1 µ L 50 ROX Reference Dye II * * in 50 µL qPCR system

The protein has a predicted MW of 31 kDa