Murashige and Skoog (MS) Medium w/ Vitamins, Sucrose, and GelzanContains Macroelements, Microelements, and Vitamins as described by Murashige & Skoog (1962), modified to contain 30 g L D Sucrose and 2 g L Gelzan. Add Cytokinins and Auxins as needed

Bacteria negative for beta-Galactosidase remain white

Jensen's Medium contains (g/L): 1g Calcium phosphate

Containing only raw materials with a purity of ≥98%

1 mM Calcium Chloride

2 g mix to make 100 mL of 1000X stock solution

or in aqueous solutions at pH 2 and pH 11

High quality YEB Agrobacterium Growth Medium contains Beef Extract

It has even been observed to trigger pathogenesis genes and Hydrogen peroxide accumulation to keep plants healthy

See ”Agrobacterium-Mediated Plant Transformation: the Biology behind the ‘Gene-Jockeying’ Tool” on PMC

2% Sodium Deoxycholic acid

Forms clear gel and suitable to see root growth in medium

immunodiffusion